Daniele De Rossi and Patrik Schick have both been passed fit to be included in the squad for Sunday's game against Benevento - while three Primavera players have also received first call-ups to the matchday squad.
De Rossi and Schick both return to availability after recent absences, although it remains to be seen if either is fit to start the game at the Stadio Olimpico.
With Radja Nainggolan and Lorenzo Pellegrini both suspended, and defender Bruno Peres omitted from the squad for disciplinary reasons, Primavera prospects Devid Bouah, Andrea Marcucci and Alessio Riccardi have been named in the 21-man squad.
See the full squad list below.
AS Roma squad to face Benevento:
Alisson Becker
Lukasz Skorupski
Bogdan Lobont
Juan Jesus
Aleksandar Kolarov
Elio Capradossi
Federico Fazio
Devid Bouah
Kostas Manolas
Kevin Strootman
Daniele De Rossi
Alessandro Florenzi
Gerson
Alessio Riccardi
Andrea Marcucci
Diego Perotti
Edin Dzeko
Patrik Schick
Cengiz Under
Gregoire Defrel
Stephan El Shaarawy
Source: As Roma